0253 Consumption of a Western diet impacts sleep microstructure during normal sleep and recovery sleep – a randomized crossover trial

Abstract Introduction While intake of specific macronutrients has been associated with changes in sleep parameters humans – such as macrostructure direct interventional evidence is lacking. Thus, we conducted a randomized trial to examine how consumption an unhealthy, Western diet impacts at the microstructure level humans. Methods In crossover study, 15 healthy normal-weight men consumed two isocaloric diets random order: unhealthy high-fat, high-sugar diet, and healthy, low-fat, low-sugar diet. Following each week-long in-lab was recorded using polysomnography (PSG), during full night sleep, after recovery occurring extended wakefulness. Sleep duration, (sleep oscillatory pattern slow waves) were investigated machine learning-based algorithms. Results duration did not differ across diets, based on actigraphy PSG. similar one week Compared resulted reduced delta power slow-wave amplitude, but increased alpha theta power, NREM sleep. Furthermore, also impacted direction Conclusion Short-term modulates restorative properties these persisted even into Whether can mediate adverse health outcomes warrants further investigation. Support (if any) This work supported by Swedish Society for Medical Research (SSMF), Council, following foundations: Diabetesfonden (2019-489), Diabetes Wellness Sverige (25-2231), Familjen Ernfors, Göran Gustafsson (2019-1941), Selander, Cancer Foundation (19 0269 Pj); Brain (FO2022-0704). The study sponsor/funding agencies involved design study; collection, analysis, interpretation data; writing report; impose any restrictions regarding publication report.